Wednesday, May 6, 2020
Juvenile Delinquent Free Essays
Juvenile Delinquent Renee Washington American Intercontinental University ? Abstract This report is in regards to our young people today that need to be helped to a point that they have meaning in their lives. There are ways that can be presented to insure that young offenders can work through the pain, and rage surrounding their emotions which causes negative behavior. Healing programs have been a recommendation thru the court systems to analyze juvenile delinquency, and find the factors that lead to meaningful resolutions. We will write a custom essay sample on Juvenile Delinquent or any similar topic only for you Order Now ? Introduction Many of our young people have lost their way in this land of democracy. Why are the statistics so high regarding juvenile delinquency, and a young male, or female appears in our courts every single day before a judge for crimes committed against other people, someoneââ¬â¢s property, or belongings that have been vandalized. Where are the families to these lost children? What causes them to erupt into crimes of violence, and mayhem? Something has to be done to help juvenile delinquents reflect on their lives, and the criminal offenses they commit. Illustrate Offenses The attitude that leads young people toward a cycle of negative behavior comes from deep rooted emotional rage, and pain which causes them to retaliate by committing offenses to others through stealing, vandalizing property, and sometimes violence against other people physically. The courts have begun to intervene on behalf of the juvenile delinquents by recommending healing programs that have been put in place to analyze juvenile delinquency, and find the factors that will lead to a resolution. Tell Causes The individual history of being betrayed by someone they trusted who cared for them as a young child. They were often neglected, abused, and victimized by a parent, or caregiver. A large number of family factors are associated with juvenile delinquency. Although it doesnââ¬â¢t excuse the offenderââ¬â¢s behavior it does help to explain why so many young boys, and girls naturally vulnerable, and instinctively trusting become victims at the hands of his, or her own parent, and their whole life begins to unravel. Without positive intervention the child is at risk of violating the law at an early age, and charged as a juvenile delinquent. Consequences/Outcome A parent that has been abused or rejected will eventually lead to emotional wounds within the souls of their children. These wounds can be painful, and traumatic to the child because of where they originated from a family member, or loved ones. Without getting the specified, and intentional healing the infection from the wounds will manifest into bitterness, rage, and pain which will lead toward delinquent patterns. Conclusion Juvenile delinquency is one of the major problems being recognized and dealt with regarding the youth in todayââ¬â¢s society. In ââ¬Å"An Update on the Cycle of Violenceâ⬠, by Cathy S. Widom, and Michael G Maxfield, (2001), stated that children being victims of abuse, and neglect in all likelihood will be arrested as juveniles if a positive intervention of hope doesnââ¬â¢t present itself in their lost lives. The Bethesda Family Services Foundation provides the type of programming needed for the juvenile delinquent, and his, or her family to start having hope toward healing. ? References Herbst, Dominic P. Helping Juveniles Reflect On Their Lives and Criminal Offenses. Corrections Today, Jun2005, Vol. 67 Issue 3, p22-25, 3p Retrieved October 30, 2012 From MasterFILE Premier Widom, Cathy S. ; Maxfield, Michael G, An Update on the Cycle of Violence Research Brief How to cite Juvenile Delinquent, Essay examples
Tuesday, May 5, 2020
Project Management for Marketing of Training
Question: Discuss about the Project Management for Marketing of Training . Answer: Introduction The project involved launch, management and analysis of the marketing campaign run for the promotion of a training workshop over major social media platforms including Facebook and Twitter. This project was for an organization that was into providing corporate workshops and training to corporate organizations. Project Scope Management Scope Statements Project Objective: Objective of the project is to manage two marketing campaigns on Facebook and on Twitter for promoting the workshop that is to be conducted one month later. The campaign would run for 20 days seeking online registrations from interested students. In this paper, stakeholders of this project would be identified and their priorities would be defined on the basis of their interests. To explain the complete process of stakeholder management, stakeholder matrix, decision matrix, communication matrix and stakeholder assessment matrix would be prepared for this project. The report would also cover agenda for stakeholder and project meetings required for project progress assessment and management(Bright Hub Media, 2015). A scope checklist if provided here to provide an insight into what would be involved on the project. Scope Checklist Project Objectives Plan for managing marketing campaigns Deliverables Stakeholder Analysis Stakeholder Identification Stakeholder Prioritization Matrix Stakeholder Matrix Decision Making Matrix Stakeholder Assessment Matrix. Communication Matrix Project Meeting Agenda(BIS, 2010) Stakeholder Analysis Stakeholders are important to analyze as they affect the outcome of the project as well as need to give an acceptance for the outcome delivered. Stakeholder analyses begin with planning and defining of its purpose. Once the purpose of project is clear, company policies required to complete project would be identified. For any project to be completed, people have to have the right skills and thus, the next step would be to hire the human resource that is capable of managing marketing campaigns for the event organization(Bright Hub Media, 2013). Next, the stakeholders have to be identified and information that is important to understand about them would be recorded such as their positions and interests which would be collected by interviewing various stakeholders of the project. A structure process may be used to collect this information and the same would then act as an input for making the project plan(Caltrans, 2007). The table below identifies some of the internal and external stakeholders of the marketing project who are affected by the project process or by its results. Project Stakeholders Internal External Affected by project process Project owner Project Sponsor Training manager Training content developers Employees Stakeholders Trainers Trainers Partners Government Agencies Client organization Workshop attendees Media Competitors Affected by project result Employees Project Sponsor System users Client Public Training Community( Changefirst Limited, 2009) Stakeholder Identification and Prioritization Matrix WHAT IS IMPORTANT TO THIS STAKEHOLDER? Project Owner Project Sponsor Trainees Trainer POWER Highest (10) Higher (9) Moderate (6) Moderately Low (5) INTEREST Higher (9) High (8) High (8) Moderately Low (5) INFLUENCE High (8) Higher (9) Moderately High (7) Very Less (4) IMPACT Highest (10) High (8) Moderate (6) Moderate (6) URGENCY Medium level (6) Medium Level (6) Moderately Less (5) Moderately High (7) LEGITIMACY High (8) Medium level (6) Less (3) Very Less (1) TOTAL 51 46 35 28 PRIORITY (KEY OR OTHER) (City of Chandler , 2000) Project Decision Making Matrix PersonMethod When Sponsor Budgeting, Funding, Critical decisions Project Manager Project Planning and critical decisions Project Owner Content planning venue planning , training schedule Core team has discussions and project manager takes a decision Inputs from team members are needed Project Team Agreement Team must have unified decision to follow Recommendations from team members Investigation needed to be done upon receiving team inputs. Decision by Team members Decision needed to be taken upon receiving team inputs(FME, 2014). Stakeholder Engagement Assessment Matrix Stakeholder management is required for engaging stakeholders which is done by understanding their needs and intersects and study the impacts that they can have on the project such that a clear action plan can be made on how to interact with them to seek maximum support for the smooth execution of the project. The responsibility to engage stakeholders would be on project manager, the level of this engagement for every stakeholder and at different project stages would vary. Stakeholder Engagement It is important that a project manger identifies all communication needs of stakeholder such that a right level of engagement may be planned in order to attain the delivery of the project successfully. The needed level of the engagement for each type of stakeholder is defined in the table below: Stakeholder Unaware Resistant Neutral Supportive Leading Project Owner C C D C D D C Project Sponsor C C C D C C Trainees D D C D D Trainers D D C C D Project Manager C D C D D C(PMI, 2015) Communication Matrix for Stakeholders Purpose: The communication plan is prepared to identify methods that can be utilized for communicating project related information with its stakeholders alongside the requirements of the project milestones enabling establishing of project monitoring and control(Pearson, 2000). Audience: Project stakeholders are the audience of the communication matrix that include project owner, trainer, trainees, project sponsor, project manager, and clients. Approach: Communication plan can be developed as per the project organization hierarchy and the allotted budget for the execution of the project. The stakeholder management process also needs an understanding of the communication frequencies required for communicating with each stakeholder. For instance, a project sponsor would need to be communicating once at the start of the project to get approval and while releasing the funds required for management of the marketing campaign. A weekly status mail on the progress of marketing may also be sent to these stakeholders. If any problems or challenges are faced during the project, the same must be communicated to concerned stakeholder by the project manager. For instance, if the venue suggested suddenly faces a problem such that it cannot be used anymore then all the stakeholders have to be communicated about the change of the training venue(Project Management Institute , 2000). Role Responsibilities Project Owner Project Planning, project monitoring, Project control, risk management, Project Sponsor Approving the funds as per the budget requirement Project Manager Project Planning, project monitoring, Project control, risk management, Progress reporting, Hiring, team management, budget approvals, deliverables, project closure Trainees Give inputs on content requirement for training and make bookings of event Trainer Provide content and delivery as per the required quantity Training Manager Select trainers, hire trainers, identify training requirements, identify marketing requirements(SAID, 2015) Project Meeting Meeting Agenda Project Name: Marketing Project Team Meeting Date: Jan 31, 2017 Time: 3:00pm-6:00pm Invitees: Jackie, John, Shin Chan Agenda Items Meeting Objective: To discuss the project status updates for the marketing of the training workshop event and come up with solutions to overcome challenges or issues being faced. Topics to cover: Review Project Progress against the plan Review if Project can be completed within the allotted Budget Review Project Progress against milestones and identify deviations Identify issues faced and come up with solutions Suggest new marketing ideas for adding platforms to digital marketing for increasing registrations Status Updates on the project Action Items(Team FME, 2014): Hire more people for managing the marketing of the event on different digital media. Project manager has to meet the owner to take a decision on possibility of adding a marketing platform Issue Log Name Date Opened Origination Potential Impact Progress Trainers took time to confirm their presence in the event 01-Jan-2017 Project Manager Delay can be caused New trainer maybe approached and hired Delays in approvals from top management 10th Jan 2017 Project Sponsor Delay on the start of marketing Take approval on urgent basis(University of Exeter, 2015) References Changefirst Limited, 2009. Change and project management , s.l.: ChangeFirst. BIS, 2010. How to organise, plan and control Projects, s.l.: BIS. Bright Hub Media, 2013. 5 Leadership Secrets of Successful Project Managers. [Online] Available at: https://www.brighthubpm.com/resource-management/122821-five-leadership-secrets-of-successful-project-managers/ Bright Hub Media, 2015. Constructing Powerful Scope Statements. [Online] Available at: https://www.brighthubpm.com/project-planning/57950-example-and-evaluation-of-project-scope-statements/ Caltrans, 2007. Project Communication Handbook. s.l.:Caltrans. City of Chandler , 2000. Project Management Methodology Guidelines, s.l.: City of Chandler . FME, 2014. Project Human Resources Management, s.l.: FME. Pearson, 2000. Basic Skills for Project Managers, s.l.: Pearson Education. PMI, 2015. Capturing the Value of PROJECT MANAGEMENT, s.l.: PMI. Project Management Institute , 2000. A guide to the Project Management Book of Knowledge , s.l.: Project Management Institute . SAID, 2015. Major Projects Leadership Academy handbook, s.l.: Cabinet Office. Team FME, 2014. Human Resource Management: Project Skill, s.l.: FME. University of Exeter, 2015. C5: Post Project Appraisal and Evaluation. [Online] Available at: https://www.exeter.ac.uk/finance/cpp/appraisal/appraisalandevaluation/
Wednesday, April 1, 2020
Mood Disorders in The Yellow Wallpaper Essay Example
Mood Disorders in The Yellow Wallpaper Essay The Yellow Wallpaper is about a husband trying to help his wife deal with her emotional disorderâ⬠as best as he can. The novel allows the reader to witness woman being driven to madness by a Victorian rest cure, a once frequently prescribed period of inactivity thought to cure female hysteria, depression, nervousness and anxiety. In the period of which this specific piece of literature was written, women had minimal rights, even concerning their mental status and rights. There were instances where not having a menstrual cycle was considered abnormal and a symptom of insanity. Symptoms such as depression after the death of a loved one, use of foul language were also reasons a woman would be admitted. Haney-Peritz, Janice. ââ¬Å"Monumental Feminism and Literatureââ¬â¢s Ancestral House: Another Look at ââ¬ËThe Yellow Wallpaper.â⬠Short Story Criticism 62. (2003): 95-107. Literature Resource Center. Web. 11 Nov. 2009. (hysteria) As she remains in the room, she begins to slip into depressive psychosis. She begins to see a woman trapped in the yellow wallpaper. The story concludes with the woman circling the room, now completely immersed in her mental illness, removing the wallpaper and stepping over her unconscious husband who had fainted at the realization of his wifes mental state. The feminism literary views show how the piece suggests patriarchal ideology and how it proves itself in 19th-century marriage and medical practice. The wallpaper itself is a metaphor whereas the nameless wife is herself trapped just like the woman she sees in the wallpaper. She could be seeing herself in the wallpaper and tries to free herself by ripping the wallpaper off the walls. She is trapped behind her husband, with no rights and no say she is essentially a Guinea pig for him. The woman inside the wallpaper represents the pervasive and inescapable injustice, much like the rules the wife hides behind. We will write a custom essay sample on Mood Disorders in The Yellow Wallpaper specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Mood Disorders in The Yellow Wallpaper specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Mood Disorders in The Yellow Wallpaper specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The wife in the story is the embodiment of struggle
Saturday, March 7, 2020
Willamette Meterorite essays
Willamette Meterorite essays When a star falls from the sky, it leaves a fiery trail. It does not die. Its shade goes back to its own place to shine again. The Indians sometimes find the small stars where they have fallen in he grass(Kohnen). The Willamette Meteorite is an extremely rare scientific example. The meteorite is made of nickel iron, and it weighs over 15.5 tons. It is 3.1 meters long, 2.1 meters wide, and 1.3 meters tall. This meteorite is the largest to be found in the United States, and is the sixth largest in the world. Most meteorites are formless lumps, but the Willamette Meteorite has huge craters all over the surface The nickel iron rock is a very rare kind of meteorite and is part of a class that comprises of about 600 out of a total of 25,000 meteorites so far found on the Earth's surface(AMNH Cullman Hall). Around 4.5 billion of years ago, a planet that was orbiting the sun broke into millions of pieces. Part of these fragments was the Willamette Meteorite. This planet was composed of an iron-nickel core, and the planet breaking up and cooling is evident within the meteorites microscopic make-up. After spending a long time orbiting in space the Willamette meteorite was involved in two large impacts. These impacts caused the meteorite to be re-heated and recrystallized. One of these collisions was probably the cause of the Willamette Meteorite to fall to earth. The meteorites first Earthy journey took it from Idaho to the Willamette Valley in Oregon(Kohnen). Around 13,500, when the Cordilleran Ice Sheet formed it lifted the meteorite, and when it melted it caused a flood that carved out the Oregon countryside, and left the meteorite in the Willamette Valley. Not in space, but after the long- term exposure and weathering the large cavities were formed. This occurred from interaction of rainwater with iron sulfide deposits in the meteorite, producing weak sulfuric acid. The etching by this ac...
Thursday, February 20, 2020
Export strategy Essay Example | Topics and Well Written Essays - 2500 words
Export strategy - Essay Example However, Jasmine rice has no immediate substitutes and it would not be possible also to create any substitute for it in foreseeable future. But that does not automatically guarantee success as there must be in place proper and rational product strategy, business process strategy, operations strategy and financial strategy to augment the unique selling point of Jasmine rice. This paper deals at length on these strategies and how they should be formulated such that success is assured. Further, this paper also tends to look into the future and try to forecast whether United Kingdom would still remain as attractive an export market it is now a decade later. After making a substantive analysis of British economy the paper concludes that though other varieties of rice produced by Siam Rice Co. might face strong competition from indigenous substitutes, Jasmine rice would still retain it unassailable position in British markets. Export Strategy Export is not the same as selling in a domestic market as it involves not only foreign currency but negotiating a foreign market, foreign tastes and preferences, foreign customs and of course foreign rules and regulations. Thus, export can only be successful if a proper export strategy is in place and is executed efficiently. It should begin with the chances of success a product might have in an export market. The thumb rule for gauging the possibilities of success is whether the product is successful in the domestic market. If a product is successful in domestic market then there is fair chance that it would be a success in foreign markets too. But care must be taken to factor in the customs, tastes and preferences prevalent in foreign markets before blindly assuming that domestic success is failsafe proof of the product being successful in foreign markets also. As for example beef from Chicago is famous for its taste across the world but if a beef exporter feels that they would be able to storm the non-vegetarian markets in In dia they would be in for a rude shock as beef is a taboo for Hindus who form nearly eighty five percent of Indian population. Similarly while pork is an all time favorite in China it does not have any market in Muslim countries where it is a forbidden food. So, an exporter needs to do elaborate market research for accurately gauging the market potential for the product they would like to export (Credit Research Foundation 2007). But before the decision to export is to be made the company must determine in no uncertain terms what it stands to gain from exporting and whether it has sufficient resources at its command to undertake the additional production quantities that would have to be done in order to service both domestic and export markets. The other issue that needs to be clarified is whether the costs related to export are less than the benefits that the company expects to garner from export activity. Only if all these three issues provide a positive answer should a company und ertake exporting activities. Else, it would be better off transacting in domestic markets only (World Export Development Forum 2007). Product Strategy As already discussed, the first test of whether a product can be exported is whether it has a stable market within the country. But, as already been
Tuesday, February 4, 2020
The Internal Business Process Perspective SLP Essay
The Internal Business Process Perspective SLP - Essay Example In a bid to move towards attaining its vision of being the leading world class financial institution, Octawise Inc. has put in place quality standards that ought to guide its internal operations. This in turn leads to lower complaints and dissonances by the customers, increased satisfaction, growth of the company in the long run and increased return on investment. As spelt out in its strategy, its attainment of market leadership position within business areas, and focus on excellence is enhanced by its efforts to put in place quality standards and measures. (B) Internal Communication Lines: Internal communication structures and processes are of critical importance in the success of any organization. Open communication lines enhance faster permeation of communication, ideas and opinions. Open communication structures also result in little distortion and misinterpretation of communication. Any organization that takes customers to be the center for its success ensures that communication lines and structures support quicker permeation of information through open communication structures, which also support innovation within an organization. Octawise Inc. encourages open communication throughout the company. This portrays Octawise Inc. as receptive to customersââ¬â¢ ideas and easy to work with Organization. Organizations with complex and closed communication lines are perceived by customers as bureaucratic and less receptive to ideas and to a greater extent hinder innovation due to likely blockage of ideas. (C) Employment and Recruitment Processes: It means little to have excellent communication lines and standards and yet have a leaking employment and recruitment process and structures which results in having wrong people in the organization. Whitwood (2011) identifies the significance of indirect processes (recruitment and placement processes) in supporting direct operations performance. Octawise Inc. has all its stages of
Monday, January 27, 2020
Bio-ethanol Production by Using Oil Palm Frond Juice (OPFJ)
Bio-ethanol Production by Using Oil Palm Frond Juice (OPFJ) OBJECTIVES To enhance bioethanol production using OPFJ as substrate To optimized biotehanol production using various fermentation mode INTRODUCTION AND RESEARCH BACKGROUND Brazil and USA is the first producers with the world ethyl alcohol production about 51000 mills liters with an average of 73% of produces ethanol worldwide corresponds to fuel ethanol, 17% to beverage ethanol and 10% to industrial ethanol (Eufrozina NIGA, 2009). Bio-ethanol can be a product from an edible source which known as first generation bio-ethanol such as sugarcane and corns. Second generation of bio-ethanol is from lignocelluloses biomass is from non-edible source such as palm oil biomass. This make second generation of bio-ethanol is a better choice to replace fossil fuel without disturbing food sources. By converting the waste into valuable product we can reduce the environmental problem. Lignocelluloses material contain about 50% 60% carbohydrate in the form of cellulose and hemicelluloses which may be fermented to ethanol and 20%-35% of lignin (Mats Galbe et al, 2007). Nowadays palm oil tree is one of the main source for the production of bio-ethanol but there are not m uch attention is been given to oil palm sap which are product that come from the tree component such as oil palm trunk and oil palm fronds. Malaysia is the worldââ¬â¢s largest exporter of palm oil product. Malaysia produces a large amount of agro-industrial residue with oil palm industry itself produced about 33 million tons of residues annually in the form of empty fruit bunch, fiber and shell (MPOB, 2009; Mohamed and Lee, 2006). Malaysia has produced about 51 million tons of oil palm fronds with 53% of the total palm biomass in year 2008 (Goh et al, 2010; MPOB, 2009). Bio-fuel that produces from palm oil tree is an environmental friendly therefore many interest are being shown to this sources. Mostly, the major parts of the solid biomass from the oil palm tree are being leave behind on the plantation is to be found as oil palm fronds. It has been reported that about 46837K tons of oil palm fronds has been produced in Malaysia in the year 2007 as an agriculture wastes. Palm oil frond is one of the useful raw materials for the production of bio-ethanol which is environmental friendly way. Pre-treatment of the oil palm frond need to be done to achieve a good bio-ethanol production. However, ethanol production from lignocellulosic biomass is relatively expensive because of the latest technologies. The main contradict are low yield and cost of the hydrolysis process (Sun and Cheng, 2002). Thus, oil palm frond juice is being introduced as another source for the production of bio-ethanol. Previous study has been shown that oil palm juice is suitable to used as fermentation feedstock because there was no inhibition on microbial growth or product formation, there were no impurities, it was easy to be operated, and it had no risk on health and safety (Zahari et al, 2012). Oil palm frond sap has been proved by Zahari et al. (2012) contains high sugar content which is 76.09 à ± 2.85 g/l. There has been reported by Eze and Ogan (1988) that sucrose is the dominant sugar in the oil palm frond sap that consist of 10% w/v, as for glucose and fructose consist of et al, 2010. However in Malaysia, a study shows that glucose is the dominant sugar in the oil palm sap (Kosugi et al., 2010). Since there are many studies show that oil palm juice contains higher sugar content, a high level of the production of bio-ethanol from oil palm sap is further study. A study by Nwachukwu (2008) shows that improving yeast resistance by protoplast fusion increased yields of ethanol by 16% v/v. Apart from that, types of fermentation also one of the factor that improve the production of bio-ethanol. Thus, this project aim is to increase the yield of the bio-ethanol production using oil palm frond juice by various type of fermentation process. PROBLEM STATEMENT Uses of natural sources like petroleum is been used over the decades and the demand of this sources are being increases from time to time. Over the century, there are an increasing of energy consumption with the increasing of world population, thus more growing countries become industrialized which there are possibility that the sources will be depleted one day. Apart from that, petroleum is harmful to human and environment and with the increasing of fossil fuel will lead to increasing of carbon dioxide that eventually leads to global warming issue. Thus, an alternative sources of the fuels is been quest to overcome the shortness of the fossil fuels. Bio-ethanol is one of the solutions to this problem. This is because bio-ethanol is a form of renewable energy source which are easy available, low cost and most efficient bio-fuel. Bio-ethanol offers a great advantages because it can be produced from various feedstock such as corn, sugarcane, red seaweed part sugar beet and many more (M ohd Dinie et al, 2013).Apart from that , bio-ethanol help in reducing air pollution and carbon dioxide accumulation. Nowadays, high demands of fuel are been constantly increase over the world. Brazil is been known as the largest producer of the sugarcane and a most competitive producer of the bio-ethanol in the world. The arises of bio-ethanol from sugarcane is a prove that energy sources are being run out and constant increasing of fuel cars around the world. Fossil energy gives an impact to the atmosphere because the burning of the petroleum result in increasing of carbon dioxide emission to the environment which is the main causes of greenhouse effect. Production of bio-ethanol is increase significantly because many countries are looking for reducing oil imports, increasing rural economies and for better air quality (Eufrozina NIGA, 2009). 4.0à METHOD 4.1à Raw material preparation and Juice Clarification. (Potential Utilization of Sap from Oil Palm (Elaeis guineensis) for Lactic Acid Production by Lactobacillus casei by S. Chooklin et al, 2011) Oil palm frond (OPF) will be harvest from oil palm tree from a local oil palm plantation. The leaves from the frond will be cut off and discard. The harvest OPF will be press using sugar-cane machine press as soon as possible after the frond was harvested. OPF juice produced will be collect and filtered using coffee filter. The filtered OPF juice will be centrifuged at 10,000 rpm for 15 minutes. Supernatant will be stored at -20à °C before use. 4.2 OPF Juice Sugar Composition (Ethanol Production Using Immobilized Saccharomyces cerevisiae in Lyophilized Cellulose Gel by Eleonora Winkelhausen et al, May 2010) HPLC type:HPLC (Shimadzu Japan ), equipped with refractive index Detector Type of column: APS-Hypersil column ( diameter of 250mm x 46mm) Mobile phase:70% Acetonitrile and 30% de-ionized water Column temperature:40à °C with maximum operating temperature of 80à °C Flow rate: 0.6 ml/min Sample volume: 20à µl Pressure: 10MPa 4.3à Pure Culture Establishment (Study on Bioethanol Production from Oil Palm Trunk (Opt) Sap by Using Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) (Isolation of Microorganism from Oil Palm Sap by Nurul Nadia Ummira, 2011) 100mL of the Nutrient Broth Medium supplemented with g/L; Yeast Extract, 5; Peptone, 10 and 15% of glucose solution, 50 (Jamaludin, 2010) will be measured in a 250mL conical flask and will be autoclaved at 121 C in 15 minutes. Saccharomyces cerevisiae will be subculture into the Nutrient Broth. The mixture will be incubated for 24 hours at 150 rpm, 30oC. The suspension obtained will be subculture on the Nutrient Agar slant and will be incubated for another 2-3 days. The Nutrient Agar slants will be stored in refrigerator at 4oC. 4.4 Inoculums preparation (Study On Bio ethanol Production From Oil Palm Trunk (OPT) Sap by Using Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) (Optimization study of ethanol fermentation from oil palm trunk, rubber wood and mixed hardwood hydrolysates using Saccharomyces cerevisiae by K.L. Chin et al , 2010) About 3 4 loop of pure culture will be transferred from agar slant into 100 mL of sterile Nutrient Broth in 250 ml conical flask plug with sterile cotton. The flask will be incubated for 24 hours at 30à °C and 150 rpm until reach standard initial concentration. The cell concentration will be standardized to 0.2 0.4 g/L (OD = 4.2) determined by turbidimetry at 600 nm. 4.5 Fermentation a) Batch fermentation will be performed for the optimization of bio-ethanol production. b) 20% (v/v) of inoculums size will be inoculated into the bioreactor with the condition of pH 4.5 and temperature of 30à ºC , air flow rate of 1 vvm, agitation of 200 rpm with the initial 02 concentration of 30 %. c) The fermentation process will be carried out for 42 hour and sample will be taken out every 3 hour and analyze for bio-ethanol production, sugar and cell biomass. d) All experiment will be run in triplicate. 4.5.1à Batch fermentation. 4.5.1.1à Effect of sugar concentration (Effect of Cultural Conditions on Ethanol Production by Locally Isolated Saccharomyces Cerevisiae Bio-07 by Arifa Tahir et al, 2010) OPF juice volume use will be varied in the range of 30% to 50%. (30%, 40%, 50%) 4.5.1.2à Effect of agitation speed (Optimization of Fermentation Medium for the Production of Ethanol from Jaggery Using Box-Behnken Design by Mary Anupama.P et al 2010) The fermentation will be carried out at different agitation rate in the range of 125 rpm to 175 rpm. (125, 150, 175) 4.5.2à Fed Batch fermentation 4.5.2.1à Effect of feeding rate (Production of Ethanol by Fed- Batch Fermentation by Ngoh Gek Cheng et al, 2009) The fed batch fermentation will be run according to the best optimized condition from the batch fermentation condition. Substrate will be feed continuously into the bioreactor using peristaltic pump at glucose concentration (glucose concentration from OPFJ?) varying from 2 ml to 8 ml at feeding rate of 2 ml/h . (2 ml , 4 ml, 8 ml) 4.5.2.2à Effect of feed time (Production of Ethanol by Fed- Batch Fermentation by Ngoh Gek Cheng et al, 2009) a) Substrate will be feed from the interval of every one hour to three hour at feeding rate of 2 ml/h. ( 1 hour , 2 hour , 3 hour ) 4.6à Harvesting (Study on Bioethanol Production from Oil Palm Trunk (Opt) Sap by Using Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana, 2010) Each batch of fermentation will be terminated according to optimized time period. b) Sample (15 ml) will be taken out and the samples will be kept in refrigerator at 4oC before analyze for bio-ethanol production, sugar and cell biomass. 4.7à Yeast strain and it improvement (High-level Production of Ethanol during Fed Batch Ethanol Fermentation with a Controlled Aeration Rate and Non-Sterile Glucose Powder Feeding of Saccharomyces cerevisiae by Hyen-beom-seo et al, 2009) a) Saccharomyces cerevisiae will be growth on Nutrient Broth (yeast extracts, 10g/l; peptone, 20g/l; glucose, 20g/l and agar, 20g/l) slant in a 30 ml universal bottle. b) The slant culture will be exposed with UV light (6 watt, 254nm) for 15 second interval at a distance of approximately 7 cm from the slant. c) A loopful of irradiated slant culture will be streak onto a Nutrient Broth agar plate and subsequently incubated for 3 days at 27à ºC. d) After that the colonies will be incubated in Nutrient Broth medium containing 100 g/l of ethanol at 27à ºC while shaking at 100 rpm for 5 days to select resistance colonies. 4.8 Analysis method 4.8.1 Sugar content by HPLC (Oil Palm Fronds Juice as Future Fermentation substrate: A Feasibility Study by Che Mohd Hakiman Che Maail et al, 2014) HPLC type:HPLC (Shimadzu Japan ), equipped with refractive index Detector Type of column: APS-Hypersil column ( diameter of 250mm x 46mm) Mobile phase:70% Acetonitrile and 30% de-ionized water Column temperature:40à °C with maximum operating temperature of 80à °C Flow rate: 0.6 ml/min Sample volume: 20à µl Pressure: 10MPa 4.8.2à Cell dry determination (Study on Bioethanol Production from Oil Palm Trunk (Opt) Sap by Using Saccharomyces Cerevisiae Kyokai No.7 by Nina Farhana 2010) Cell suspension will be vaccum-filtered using 0.45à µm filter paper Cell suspension for each samples will have to be rinsed 2x with distilled water. Each sample will be dried at 70à °C more than 24 hours until constant weight achieved. Dried filter paper will be weighed on a analytical balance to measure the biomass. 4.8.3 Ethanol concentration using GC (Production of Ethanol by Fed ââ¬âBatch Fermentation by Ngoh Gek Cheng et al, 2009) Column : RT-Q-BOND (inner diameter of 0.32 mm) Carrier gas: Helium gas Detector: Flame ionization detector (FID) Temperature : 200 C Flow rate : 21.9 mL/min Pressure : 71.1 kPa Holding time : 5 minutes % bioethanol yield is calculated using this formula: 5.9 OPTIMIZATION OF ETHANOL FERMENTATION RSM analysis will be conducted using Design Expert Software. The optimized parameters will be verified by running the new fermentation as suggested. 6.0 ANALYSIS The significance of difference between each test variable will be determined using one way ANOVA analysis and Least Significance Test, computed using SPSS version 21.0 software. All tests will be done with a confidence interval of 95%. 7.0à REFERENCES Ngoh Gek Cheng, M. H., Andri Chahyo Kumoro, Tham, C. F. L. A. M. 2009. Production of Ethanol by Fed-Batch Fermentation. Pertanika J. Sci. Technol., 17, 399 ââ¬â 408. Hyeon-Beom Seo, Seung Seop Kim,Hyoen0Yong Lee and Kyung Hwan Jung (2009).High level Production of Ethanol during Fed-Batch Ethanol Fermentation with a controlled Aeration Rate and Non-Sterile Glucose Powder Feeding of Saccharomyces cerevisie .Biotechnology and Bioprocess Engineering,14, 591-598. Jamaludin, N. F. M. 2010. Study On Bioethanol Production From Oil Palm Trunk (Opt) Sap By Using Saccharomyces Cerevisiae Kyokai No.7(ATCC 26422). Bachelor of Chemical Engineering (Biotechnology), Universiti Malaysia Pahang. Zahari, M. A., Zakaria, M. R., Ariffin, H., Mokhtar, M. N., Salihon, J., Shirai, Y. Hassan, M. A. 2012. Renewable sugars from oil palm frond juice as an alternative novel fermentation feedstock for value-added products. Bioresour Technol, 110, 566-71. Che Mohd Hakiman Che Maail, Hidayah Ariffin,Mohd Ali Hassan,Umi Kalsom Shah and Yoshihito Shirai (2014).Oil Palm Frond Juice as Future Fermentation Substrate : A Feasibility Study.Bioresource Technology,110,566-71. Miller, G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar,Anal. Chem.,31,426, 1959. GANTT CHART AND MILESTONES
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